| Primary Identifier | MGI:7620246 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Igh |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129P2/OlaHsd)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The allele was created by first inserting an Actb-neo cassette followed by a loxP (fl) site at position 116,237,220 (mm9, Chr.12) and blasticidin resistance gene (Bsd)-rox sequence into the middle of the VH gene cluster, followed by the insertion of a frt-Pgke promoter sequence-frt and a second lox (lox71) site (in the inverted orientation; ifl) at position 117,126,667 followed by Puro cassette in the Igh 5' region. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination inverted the floxed 890 bp. |
