| First Author | Pailhoux EA | Year | 1990 |
| Journal | J Biol Chem | Volume | 265 |
| Issue | 32 | Pages | 19932-6 |
| PubMed ID | 2123194 | Mgi Jnum | J:10833 |
| Mgi Id | MGI:59278 | Doi | 10.1016/s0021-9258(17)45463-9 |
| Citation | Pailhoux EA, et al. (1990) Androgen-dependent protein from mouse vas deferens. cDNA cloning and protein homology with the aldo-keto reductase superfamily. J Biol Chem 265(32):19932-6 |
| abstractText | We report the cloning and sequencing of a 1225-base pair cDNA encoding an abundant protein from mouse vas deferens. An open reading frame of 948 nucleotides encodes a protein of 316 amino acids with a calculated mass of 35,965 Da. A high degree of homology was found between this protein and members of the aldo-keto reductase superfamily, especially aldose reductase from human placenta (82%) and from rat lens (80%), suggesting that it could be an aldose reductase. Castration resulted in a marked decrease in the level of the 1.4 kilobase mRNA coding for the 35,965 protein, whereas administration of testosterone to castrated males resulted in a marked increase. Southern hybridization analysis of mouse genomic DNA revealed relatively simple patterns of bands. |
