| First Author | Björgvinsdóttir H | Year | 1997 |
| Journal | Blood | Volume | 89 |
| Issue | 1 | Pages | 41-8 |
| PubMed ID | 8978275 | Mgi Jnum | J:38041 |
| Mgi Id | MGI:85433 | Doi | 10.1182/blood.v89.1.41.41_41_48 |
| Citation | Bjorgvinsdottir H, et al. (1997) Retroviral-mediated gene transfer of gp91phox into bone marrow cells rescues defect in host defense against Aspergillus fumigatus in murine X-linked chronic granulomatous disease. Blood 89(1):41-8 |
| abstractText | The X-lnked form of chronic granulomatous disease (X-CGD), an inherited deficiency of the respiratory burst oxidase, results from mutations in the X-linked gene for gp91(phox), the larger subunit of the oxidase cytochrome b. The goal of this study was to evaluate the impact of retroviral-mediated gene transfer of gp91(phox) on host defense against Aspergillus fumigatus in a murine model of X-CGD. Retrovirus vectors constructed using the murine stem cell virus (MSCV) backbone were used for gene transfer of the gp91(phox) cDNA into murine X-CGD bone marrow cells. Transduced cells were transplanted into lethally irradiated syngeneic X-CGD mice. After hematologic recovery, superoxide production, as monitored by the nitroblue tetrazolium (NET) test, was detected in up to approximate to 80% of peripheral blood neutrophils for at least 28 to 35 weeks after transplantation. Neutrophil expression of recombinant gp91(phox) and superoxide production were significantly less than wild- type neutrophils. However, 9 of 9 mice with approximate to 50% to 80% NBT+ neutrophils after gene transfer did not develop lung disease after respiratory challenge with 150 to 500 A fumigatus spores, doses that produced disease in 16 of 16 control X-CGD mice. In X-CGD mice transplanted with mixtures of wild-type and X-CGD bone marrow, greater than or equal to 5% wild-type neutrophils were required for protection against A fumigatus challenge. These data suggest that expression of even low levels of recombinant gp91(phox) can substantially improve phagocyte function in X-CGD, although correction of very small percentage of phagocytes may not be sufficient for protection against A fumigatus. (C) 1997 by The American Society of Hematology. |
