| First Author | Antoch MP | Year | 1997 |
| Journal | Cell | Volume | 89 |
| Issue | 4 | Pages | 655-67 |
| PubMed ID | 9160756 | Mgi Jnum | J:40363 |
| Mgi Id | MGI:87703 | Doi | 10.1016/s0092-8674(00)80246-9 |
| Citation | Antoch MP, et al. (1997) Functional identification of the mouse circadian Clock gene by transgenic BAC rescue. Cell 89(4):655-67 |
| abstractText | As a complementary approach to positional cloning, we used in vivo complementation with bacterial artificial chromosome (BAC) clones expressed in transgenic mice to identify the circadian Clock gene. A 140 kb BAC transgene completely rescued both the long period and the loss-of- rhythm phenotypes in Clock mutant mice. Analysis with overlapping BAC transgenes demonstrates that a large transcription unit spanning similar to 100,000 base pairs is the Clock gene and encodes a novel basic-helix-loop- helix-PAS domain protein. Overexpression of the Clock transgene can shorten period length beyond the wild-type range, which provides additional evidence that Clock is an integral component of the circadian pacemaking system. Taken together, these results provide a proof of principle that ''cloning by rescue'' is an efficient and definitive method in mice. |
