Primary Identifier | MGI:3831538 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Sac3d1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A SpeI-NotI fragment containing the proximal promoter and the upstream-portion of exon 1 was replaced with neomycin resistance gene. Correct targeting was confirmed by Southern blotting. Gene inactivation was confirmed by a failure to detect protein product in immunoblot analysis of thymocyte nuclear extracts collected from homozgytoes. |