Primary Identifier | MGI:3760426 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence | Gene | Tg(UBC-scFv)2Nemz |
Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | These mice are designed to express a membrane-tethered single chain variable fragment antibody with specificity to mouse Igk light chain. The 4 kb "Ulik" transgene contained the 1.2 kb human ubiquitin C promoter, rheumatoid factor Ig light chain gene leader exon and first intron, the expressed variable genes from the rat anti-k hybridoma 187 line, the hinge, CH2, and CH3 sequences from rat gamma chain 1 (CH1 domain deleted), and the transmembrane and intracytoplasmic sequences from the H2-kb gene. |