| First Author | Aizman R | Year | 2002 |
| Journal | Am J Physiol Renal Physiol | Volume | 283 |
| Issue | 3 | Pages | F569-77 |
| PubMed ID | 12167609 | Mgi Jnum | J:78904 |
| Mgi Id | MGI:2386467 | Doi | 10.1152/ajprenal.00376.2001 |
| Citation | Aizman R, et al. (2002) Generation and phenotypic analysis of CHIF knockout mice. Am J Physiol Renal Physiol 283(3):F569-77 |
| abstractText | Corticosteroid hormone-induced factor (CHIF) is a short epithelial-specific protein that is independently induced by aldosterone and a high-K(+) diet. It is a member of the FXYD family of single-span transmembrane proteins that include phospholemman, Mat-8, and the gamma-subunit of Na(+)-K(+)-ATPase. A number of studies have suggested that these proteins are involved in the regulation of ion transport and, in particular, functionally interact with the Na(+)-K(+)-ATPase. The present study describes the characterization, targeted disruption, and phenotypic analysis of the mouse CHIF gene. The CHIF knockout mice are viable and not distinguishable from wild-type littermates under normal conditions. Under K(+) loading, they have a twofold higher urine volume and an increased glomerular filtration rate. Similar but smaller effects are observed in mice fed a low-Na(+) diet. Treating K(+)-loaded mice for 10 days with furosemide resulted in lethality in the knockout mice (17 of 39) but not in the wild-type group (1 of 39). The data are consistent with an effect of CHIF on the Na(+)-K(+)-ATPase that is specific to the outer and inner medullary duct, its major expression site. |
