| First Author | Yukitake H | Year | 2002 |
| Journal | Biochim Biophys Acta | Volume | 1577 |
| Issue | 1 | Pages | 126-32 |
| PubMed ID | 12151104 | Mgi Jnum | J:78327 |
| Mgi Id | MGI:2384227 | Doi | 10.1016/s0167-4781(02)00411-6 |
| Citation | Yukitake H, et al. (2002) AMAP-1, a novel testis-specific AMY-1-binding protein, is differentially expressed during the course of spermatogenesis. Biochim Biophys Acta 1577(1):126-32 |
| abstractText | AMY-1 has been identified by us as a c-Myc-binding protein and was found to stimulate c-Myc transcription activity. AMY-1 was also found to be associated with AKAP84/149 in the mitochondria in somatic cells and sperm, suggesting that it plays a role in spermatogenesis. To access the molecular function of AMY-1, a two-hybrid screening of cDNAs encoding AMY-1-binding proteins was carried out with AMY-1 as a bait using a human testis cDNA library, and a clone encoding a novel protein, AMAP-1, was obtained. The amap-1 gene was mapped at human chromosome 17q21. AMY-1 was found to bind to and be colocalized with AMAP-1 in human 293T and HeLa cells. AMAP-1 was found to be specifically expressed in the testis and expressed post-meiotically in the testis, as was AMY-1. These results suggest that both AMAP-1 and AMY-1 play roles in spermatogenesis. |
