| First Author | Sugino H | Year | 1997 |
| Journal | Genomics | Volume | 43 |
| Issue | 2 | Pages | 209-15 |
| PubMed ID | 9244438 | Mgi Jnum | J:38472 |
| Mgi Id | MGI:85855 | Doi | 10.1006/geno.1997.4812 |
| Citation | Sugino H, et al. (1997) Genomic organization and chromosomal localization of the mouse telencephalin gene, a neuronal member of the ICAM family. Genomics 43(2):209-15 |
| abstractText | Telencephalin is a cell adhesion molecule belonging to the immunoglobulin (Ig) superfamily, whose expression is restricted to subsets of neurons in the telencephalon, the most rostral segment of brain. Of all the Ig superfamily molecules so far identified, the structure of telencephalin is most closely related to those of intercellular adhesion molecules (ICAMs)-1 and -3. Here we report the cloning, characterization, and chromosomal localization of the mouse telencephalin gene (Tlcn). The Tlcn gene spanned about 6.3 kb and consisted of 11 exons. A signal peptide and individual nine Ig-like domains of telencephalin were encoded by a single exon, while the transmembrane and cytoplasmic regions were fused in a same exon. The primer extension technique was used to establish that the transcription initiation sites were located 92-95 bp upstream from the ATG start codon. DNA sequencing of the 5'-flanking region revealed the presence of a strong initiator element for TATA-less genes, two CAAT boxes, and numerous potential transcription factor binding sites including four E-box and two N-box sequences. Interspecific backcross analysis demonstrated that the Tlcn gene was mapped in the proximal region of mouse chromosome 9 in close vicinity to the Icam-1 gene, suggesting that Tlcn and Icam-1 are derived from a common ancestral gene by gene duplication. (C) 1997 Academic Press. |
