| Primary Identifier | MGI:2674114 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cacna1c |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 14 and 15, which together encode the transmembrane IIS5 segment and the pore in repeat II, were flanked by loxP sites and subsequently excised in vitro cre-mediated recombination prior to germline transmission. Though RT-PCR revealed aberrant transcript in which exon 13 had spliced to a point within intron 16, no protein was detected in the hearts of homozygous mutant embryos by Western blot analysis. |
