| Primary Identifier | MGI:3720808 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Bmal1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using homologous recombination in a bacterial artificial chromosome (BAC, clone RPCI23-331K23), an FRT-flanked neo cassette (followed by a loxP site) was inserted immediately downstream of exon 8 of the targeted gene. An additional loxP site was added upstream of exon 8 when the targeting vector was excised from the BAC. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to Flpe-expressing mice to excise the neo cassette. |
