First Author | Hunter KW | Year | 1993 |
Journal | Genomics | Volume | 18 |
Issue | 3 | Pages | 510-9 |
PubMed ID | 8307560 | Mgi Jnum | J:16146 |
Mgi Id | MGI:64236 | Doi | 10.1016/s0888-7543(11)80007-8 |
Citation | Hunter KW, et al. (1993) Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1. Genomics 18(3):510-9 |
abstractText | Interspersed repetitive sequence (IRS) PCR and repetitive element-to-bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15, 2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high-density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross. |