| First Author | Wang Z | Year | 2019 |
| Journal | J Immunol | Volume | 202 |
| Issue | 10 | Pages | 2999-3007 |
| PubMed ID | 30952816 | Mgi Jnum | J:274894 |
| Mgi Id | MGI:6296848 | Doi | 10.4049/jimmunol.1801578 |
| Citation | Wang Z, et al. (2019) Langerhans Cells Control Lymphatic Vessel Function during Inflammation via LIGHT-LTbetaR Signaling. J Immunol 202(10):2999-3007 |
| abstractText | The lymphatic vasculature is an important route for dendritic cell (DC) or tumor cell migration from peripheral tissues to draining lymph nodes (DLNs). However, the underlying molecular and cellular mechanisms remain poorly understood. In this study, using conventional bone marrow chimeric mice and additional UVB radiation, we found that deficiency of LIGHT but not lymphotoxin (LT) alpha1beta2, likely on radioresistant Langerhans cells (LCs), resulted in impaired skin DC migration to DLNs during LPS-induced inflammation. In addition, LT beta receptor (LTbetaR), but not herpes virus entry mediator, was found to be the receptor of LIGHT controlling DC migration. Furthermore, conditional deficiency of LTbetaR in Tie2 (cre) or Lyve1 (cre) mice, but not in LTbetaR-deficient bone marrow chimeric mice, impaired DC migration, suggesting an important role of LTbetaR in radioresistant lymphatic endothelial cells (LECs), although the role of LTbetaR in blood endothelial cells remains intriguing. Mechanistically, the gene expression of both CCL21 and CCL19 was found to be reduced in skin LECs isolated from LC-LIGHT-conditionally deficient or Lyve1 (cre) Ltbr (fl/fl) mice compared with their controls upon LPS stimulation. Soluble recombinant LIGHT was able to upregulate CCL21 and CCL19 gene expression on SVEC4-10 endothelial cells. Doxycycline, an inhibitor of soluble LIGHT release in the inflamed skin, impaired skin CCL21 and CCL19 expression and DC migration. In addition, melanoma cell metastasis to DLNs was also inhibited in LC-LIGHT-conditionally deficient or Lyve1 (cre) Ltbr (fl/fl) mice. Together, our data suggest, to our knowledge, a previously unrecognized scenario in which LCs activate LECs via the LIGHT-LTbetaR signaling axis to promote DC migration or tumor cell metastasis. |
