| First Author | Kozak LP | Year | 1983 |
| Journal | Proc Natl Acad Sci U S A | Volume | 80 |
| Issue | 10 | Pages | 3020-4 |
| PubMed ID | 6407006 | Mgi Jnum | J:7078 |
| Mgi Id | MGI:55549 | Doi | 10.1073/pnas.80.10.3020 |
| Citation | Kozak LP, et al. (1983) Mouse sn-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence. Proc Natl Acad Sci U S A 80(10):3020-4 |
| abstractText | The isozymes of glycerol-3-phosphate dehydrogenase (GPDH; sn-glycerol-3-phosphate:NAD+2-oxidoreductase, EC 1.1.1.8) in tissues of the mouse are coded for by two structural genes, Gdc-1 and Gdc-2, located on chromosomes 15 and 9, respectively. In order to investigate the expression of these genes, we isolated a GPDH cDNA clone from a mRNA preparation isolated from brown adipose tissue. The GPDH cDNA clone was identified by colony hybridization and hybrid selection of a mRNA that was translated in vitro to produce immunoprecipitable GPDH protein. In blot analysis, the GPDH cDNA hybridized to a single mRNA species that migrated at the position of 23S ribosomal RNA. This GPDH cDNA clone was mapped to the Gdc-1 locus by identification of a restriction enzyme polymorphism present in genomic DNA isolated from Gdc-1 congeneic lines of mice. |
