| First Author | Loft A | Year | 2021 |
| Journal | STAR Protoc | Volume | 2 |
| Issue | 3 | Pages | 100805 |
| PubMed ID | 34527961 | Mgi Jnum | J:360067 |
| Mgi Id | MGI:7797676 | Doi | 10.1016/j.xpro.2021.100805 |
| Citation | Loft A, et al. (2021) Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing. STAR Protoc 2(3):100805 |
| abstractText | Isolation of nuclei tagged in specific cell types (INTACT) allows for stress-free and high-throughput analyses of cellular subpopulations. Here, we present an improved protocol for isolation of pure and high-quality GFP-labeled nuclei from frozen livers of INTACT mice, as well as protocols for downstream sequencing analyses. The adaptation to frozen tissue provides a pause point that allows sampling at multiple time points and/or phenotypic characterization of livers prior to nuclei isolation and downstream analyses. For complete details on the use of this protocol, please refer to Loft et al. (2021). |
