| First Author | Gounari F | Year | 2002 |
| Journal | Nat Immunol | Volume | 3 |
| Issue | 5 | Pages | 489-96 |
| PubMed ID | 11927910 | Mgi Jnum | J:76234 |
| Mgi Id | MGI:2178892 | Doi | 10.1038/ni778 |
| Citation | Gounari F, et al. (2002) Tracing lymphopoiesis with the aid of a pTalpha-controlled reporter gene. Nat Immunol 3(5):489-96 |
| abstractText | A transgenic reporter mouse strain, which expressed the human CD25 (hCD25) surface marker as a reporter under the control of the pre-T cell receptor alpha(pTalpha) promoter, was used to identify lymphoid precursors that expressed pTalpha intracellularly. The hCD25 reporter marked intra- and extrathymic precursors of lymphocytes but not myeloid cells. The earliest intrathymic precursors were CD4(lo)CD8(-)CD25(-)CD44(+)c-Kit(+) cells that expressed elevated levels of Notch-1 mRNA. Clonogenic assays showed that the extrathymic precursors were common lymphoid progenitors (CLPs) that included CD19(-), B220(+), Thy1(+) and CD4(+) cells. Thus, the pTalpha reporter can be used to trace lymphopoiesis between CLPs and alphabeta T cells. The slower extinction of the hCD25 reporter compared to pTalpha enabled us to define points at which pTalpha(-) lineages branched off. |
