| First Author | Nambu A | Year | 2006 |
| Journal | Int Immunol | Volume | 18 |
| Issue | 5 | Pages | 701-12 |
| PubMed ID | 16569679 | Mgi Jnum | J:108548 |
| Mgi Id | MGI:3624226 | Doi | 10.1093/intimm/dxl007 |
| Citation | Nambu A, et al. (2006) IL-1{beta}, but not IL-1{alpha}, is required for antigen-specific T cell activation and the induction of local inflammation in the delayed-type hypersensitivity responses. Int Immunol 18(5):701-12 |
| abstractText | As IL-1 expression is augmented in delayed-type hypersensitivity (DTH) responses, we analyzed the role of IL-1 in this response. DTH responses against methyl BSA (mBSA) were significantly suppressed in IL-1beta-deficient (IL-1beta(-/-)) and IL-1alpha/beta(-/-) mice, but not in IL-1alpha(-/-) mice. In contrast, responses in IL-1R antagonist(-/-) (IL-1Ra(-/-)) mice were exacerbated. Lymph node cells derived from mBSA-sensitized IL-1beta(-/-), IL-1alpha/beta(-/-) and IL-1R type I (IL-1RI)(-/-) mice, but not from IL-1alpha(-/-) mice, exhibited reduced proliferative responses against mBSA, while these from IL-1Ra(-/-) mice demonstrated augmented responses. DTH responses in wild-type mice following adoptive transfer of CD4(+) T cells from mBSA-sensitized IL-1alpha/beta(-/-) mice were also reduced, while those in mice given cells derived from IL-Ra(-/-) mice were increased. DTH responses in IL-1RI(-/-), but not IL-1alpha/beta(-/-), mice were reduced upon transplantation of mBSA-sensitized CD4(+) T cells from wild-type mice. The recall response of mBSA-sensitized CD4(+) T cells against mBSA decreased upon co-culture with dendritic cells (DCs) from IL-1RI(-/-) mice, while the responses were normal with DCs from IL-1alpha/beta(-/-) mice. DTH responses in tumor necrosis factor alpha(-/-) (TNF(-/-)) mice were also suppressed; the magnitude of the suppression in IL-1alpha/beta(-/-)TNF(-/-) mice, however, was similar to that observed in IL-1alpha/beta(-/-) mice. These observations indicate that IL-1 possesses dual functions during the DTH response. IL-1beta is necessary for the efficient priming of T cells. In addition, CD4(+) T cell-derived IL-1 plays an important role in the activation of DCs during the elicitation phase, resulting in the production of TNF, that activate allergen-specific T cells. |
