| Primary Identifier | MGI:5490865 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter | Gene | Rho |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The endogenous gene was replaced with an HPRT minigene and a human RHO gene with a P23H mutation and a C terminal fusion to EGFP via homologous recombination. Cre mediated recombination removed the HPRT minigene. Expression levels of the fusion protein are reduced compared to wild-type protein expression and much of what is expressed is degraded. Most of the fusion protein is mislocalized to the inner segments and outer nuclear layer of the retina. |
