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Allele : Gt(ROSA)26Sor<tm2.1(CAG-HIST1H2BB/EGFP)Zjh> gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Z Josh Huang
Primary Identifier  MGI:6741027 Allele Type  Targeted
Attribute String  Conditional ready, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a FRT-flanked STOP cassette, an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by an enhanced green fluorescent protein sequence), and a Neo-polyA cassette. The entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. Cre mediated recombination removed the loxP-flanked STOP cassette. This alle was generated by crossing mouse with parental Gt(ROSA)26Sortm2(CAG-HIST1H2BB/EGFP)Zjh to cre-deleter mouse. It is an flp reporter.
  • mutations:
  • Insertion
  • synonyms:
  • H2B-GFP,
  • H2B-GFP
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1 Feature

Trail: Allele

Genome

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Phenotype

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