Primary Identifier | MGI:3723138 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Eqtn |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to replace exons 4-7 with a neomycin resistance cassette and a thymidine kinase (TK) gene. Western blotting revealed the complete absence of protein in homozygous mutant testes. Immunohistochemical analysis and immunofluorescence confirmed the absence of detectable protein in homozygous mutant sperm. |