| First Author | Okamura H | Year | 2013 |
| Journal | FEBS Lett | Volume | 587 |
| Issue | 1 | Pages | 48-53 |
| PubMed ID | 23183242 | Mgi Jnum | J:193136 |
| Mgi Id | MGI:5467693 | Doi | 10.1016/j.febslet.2012.10.041 |
| Citation | Okamura H, et al. (2013) Protein phosphatase 2A Calpha is involved in osteoclastogenesis by regulating RANKL and OPG expression in osteoblasts. FEBS Lett 587(1):48-53 |
| abstractText | We examined whether alteration of PP2A Calpha expression in osteoblasts is involved in osteoclast differentiation. Reduction of PP2A Calpha in MC3T3-E1 cells (shPP2A) decreased receptor activator of nuclear factor kappaB ligand (RANKL) expression and increased osteoprotegerin (OPG) expression. The conditioned medium from shPP2A cells failed to induce NFATc1 as well as the expression of osteoclast marker genes cathepsin K and osteoclast-associated receptor (OSCAR) in bone marrow macrophage cells. Treatment of bone marrow macrophage cells with the conditioned medium from shPP2A cells impaired osteoclastogenesis. These results suggest that alteration of PP2A Calpha expression in osteoblasts modulates the expressions of RANKL and OPG, which are involved in osteoclastogenesis via the NFATc1 transcription factor. |
