| First Author | Cieslik KA | Year | 2011 |
| Journal | Am J Pathol | Volume | 179 |
| Issue | 4 | Pages | 1792-806 |
| PubMed ID | 21819956 | Mgi Jnum | J:179946 |
| Mgi Id | MGI:5304644 | Doi | 10.1016/j.ajpath.2011.06.022 |
| Citation | Cieslik KA, et al. (2011) Defective myofibroblast formation from mesenchymal stem cells in the aging murine heart rescue by activation of the AMPK pathway. Am J Pathol 179(4):1792-806 |
| abstractText | Aged mice in a murine model of myocardial infarction exhibit less effective myocardial repair. We hypothesized that the deficiency arises from altered lineage choice of endogenous mesenchymal stem cells (MSCs) and faulty maturation of myofibroblasts. Examination of cardiac MSCs revealed a substantial reduction in the pluripotency marker Nanog in cells from aged mice. In addition, the aged MSCs demonstrated a redirected lineage choice that favored adipocytic commitment over fibroblast or myofibroblast differentiation. Fibroblasts derived from aged MSCs demonstrated reduced expression of transforming growth factor-beta (TGF-beta) receptors I and II and diminished SMAD3 phosphorylation, associated with attenuated contractility and migration. Overexpression of constitutively active TGF-beta receptor I in aged cardiac fibroblasts ameliorated their defective motility but did not improve their contractility. Culturing of MSCs and fibroblasts with AICAR (5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside) to activate adenosine monophosphate-activated kinase resulted in TGF-beta-dependent development of myofibroblasts with markedly enhanced contractility despite no reduction in adipocytic commitment or increased expression of TGF-beta receptors and SMAD3 phosphorylation. The data suggest an adenosine monophosphate-activated kinase-dependent gain of function as mediated by phosphorylation of TGF-beta-activated kinase 1 and p38 mitogen-activated protein kinase, which amplifies the response to TGF-beta1 via a non-canonical pathway, thus compensating for the reduced expression of TGF-beta receptors. |
