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Allele : Gt(ROSA)26Sor<tm4(CAG-mOrange2,-EGFP,-mKate2)Zjh> gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Z Josh Huang
Primary Identifier  MGI:5700411 Allele Type  Targeted
Attribute String  Conditional ready, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), and three farnesylated fluorescent protein sequences uniquely flanked with pairs of incompatible lox sites. Specifically, the coding region contains (from 5' to 3') a loxP site, a lox2272 site, a loxN site, a transcriptional STOP signal, mOrange2 sequence (derived from a monomeric red fluorescent protein) with polyA sequence, a loxP site, EGFP (enhanced green fluorescent protein) with polyA sequence, a lox2272 site, mKate2 (derived from a monomeric far-red fluorescent protein). This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
  • mutations:
  • Insertion
  • synonyms:
  • RGBow,
  • RGBow
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