| First Author | Krieg P | Year | 1998 |
| Journal | Biochim Biophys Acta | Volume | 1391 |
| Issue | 1 | Pages | 7-12 |
| PubMed ID | 9518531 | Mgi Jnum | J:46809 |
| Mgi Id | MGI:1202103 | Doi | 10.1016/s0005-2760(97)00214-2 |
| Citation | Krieg P, et al. (1998) cDNA cloning of a 8-lipoxygenase and a novel epidermis-type lipoxygenase from phorbol ester-treated mouse skin. Biochim Biophys Acta 1391(1):7-12 |
| abstractText | Using a combination of PCR cloning and conventional screening procedures, we isolated from phorbol ester-treated mouse epidermis two full length cDNA clones encoding novel lipoxygenases. One of the cDNAs turned out to be identical to the recently cloned 8-lipoxygenase [Jisaka et al., J. Biol. Chem. 272 (1997) 24 410-24 416], the open reading frame of the second one corresponded to a protein of 701 amino acids with a calculated molecular mass of 80.6 kDa. The amino acid sequence showed 50.8% identity to human 15-lipoxygenase 2, approximately 40% to 5-lipoxygenase and 35% to 12- and 15- lipoxygenases. A unique structural feature is the insertion of 31 amino acid residues in the amino-terminal part of the molecule. Based on these data, we conclude that this epidermis-derived cDNA encodes a novel lipoxygenase isoform termed provisionally epidermis-type lipoxygenase 2 (e-LOX 2). Copyright 1998 Elsevier Science B.V. |
