| First Author | Heim N | Year | 2007 |
| Journal | Nat Methods | Volume | 4 |
| Issue | 2 | Pages | 127-9 |
| PubMed ID | 17259991 | Mgi Jnum | J:147189 |
| Mgi Id | MGI:3839654 | Doi | 10.1038/nmeth1009 |
| Citation | Heim N, et al. (2007) Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor. Nat Methods 4(2):127-9 |
| abstractText | Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits. |
