| First Author | Soutto M | Year | 2002 |
| Journal | J Immunol | Volume | 169 |
| Issue | 8 | Pages | 4205-12 |
| PubMed ID | 12370350 | Mgi Jnum | J:79538 |
| Mgi Id | MGI:2388451 | Doi | 10.4049/jimmunol.169.8.4205 |
| Citation | Soutto M, et al. (2002) A Minimal IFN-gamma Promoter Confers Th1 Selective Expression. J Immunol 169(8):4205-12 |
| abstractText | Th1 and Th2 cells differentiate from naive precursors to effector cells that produce either IFN-gamma or IL-4, respectively. To identify transcriptional paths leading to activation and silencing of the IFN-gamma gene, we analyzed transgenic mice that express a reporter gene under the control of the 5' IFN-gamma promoter. We found that as the length of the promoter is increased, -110 to -225 to -565 bp, the activity of the promoter undergoes a transition from Th1 nonselective to Th1 selective. This is due, at least in part, to a T box expressed in T cells-responsive unit within the -565 to -410 region of the IFN-gamma promoter. The -225 promoter is silent when compared with the -110 promoter and silencing correlates with Yin Yang 1 binding to the promoter. The p38 mitogen-activated protein kinase signaling pathway, which also regulates IFN-gamma gene transcription, regulates the -70- to -44-bp promoter element. Together, the results demonstrate that a minimal IFN-gamma promoter contains a T box expressed in T cells responsive unit and is sufficient to confer Th1 selective expression upon a reporter. |
