| First Author | Larhammar D | Year | 1985 |
| Journal | J Biol Chem | Volume | 260 |
| Issue | 26 | Pages | 14111-9 |
| PubMed ID | 2997193 | Mgi Jnum | J:28386 |
| Mgi Id | MGI:76004 | Doi | 10.1016/s0021-9258(17)38690-8 |
| Citation | Larhammar D, et al. (1985) Sequence of gene and cDNA encoding murine major histocompatibility complex class II gene A beta 2. J Biol Chem 260(26):14111-9 |
| abstractText | The murine major histocompatibility complex is known to express two class II molecules, A and E. They are composed of one alpha- and one beta-polypeptide chain. Recently, we reported the finding of an additional beta-chain second domain exon tentatively designated A beta 2. We describe here the nucleotide sequence of the A beta 2 gene and an A beta 2 cDNA clone. A beta 2 displays the same exon organization as the known expressed beta-chain genes, and the predicted A beta 2 polypeptide shows all the characteristic features of the expressed beta-chains. The predicted A beta 2 polypeptide shows 49-56% amino acid sequence identity to A beta and E beta chains and to the human DP, DQ, and DR beta-chains. These are 63-79% homologous to each other, indicating that A beta 2 is the most divergent member of the beta-chain family. The A beta 2 gene seems to be transcribed in the same types of cells as other class II genes. Detection of incompletely spliced A beta 2 mRNA and the finding of a cDNA clone containing an intron segment suggest that A beta 2 transcripts are processed slowly. Hybridizations with A beta 2 probes to restriction enzyme-digested genomic DNA indicate that the A beta 2 gene displays lower allelic polymorphism than the A beta gene. |
