| First Author | Wu LC | Year | 1996 |
| Journal | Genomics | Volume | 35 |
| Issue | 3 | Pages | 415-24 |
| PubMed ID | 8812474 | Mgi Jnum | J:34609 |
| Mgi Id | MGI:82064 | Doi | 10.1006/geno.1996.0380 |
| Citation | Wu LC, et al. (1996) The mouse DNA binding protein Rc for the kappa B motif of transcription and for the V(D)J recombination signal sequences contains composite DNA-protein interaction domains and belongs to a new family of large transcriptional proteins. Genomics 35(3):415-24 |
| abstractText | Rc is a DNA binding protein with dual specificities for the V(D)J recombination signal sequences and for the B motif of the immunoglobulin kappa chain gene enhancer. The largest Re transcript present in lymphoid cells/tissues is similar to 9 kb, Molecular cloning and sequence determination for 8822 bp of mouse Re cDNA revealed an open reading frame of 2282 amino acids and long 5'- and 3'- untranslated regions. The derived amino acid sequence contains multiple DNA and protein interaction domains. Composite ZAS structures with tandem zinc fingers, an acidic motif, and a Ser/Thr-rich segment are located near the N-terminal and the C-terminal regions. The middle region of Re contains a lone zinc finger, an acidic motif, a Ser-rich region, a nucleus localization signal, and GTPase motifs. Cloning and characterization of a mouse Re gene show that the Re cDNA corresponds to seven exons located in a genomic region spanning 70 kb. Exon 2 is exceptionally large, with 5487 bp. cDNA cloning and Northern blot analyses revealed multiple Re transcripts, probably generated by alternative splicings, Sequence comparisons show that Re belongs to a ZAS protein family that is involved in gene transcription and/or DNA recombination, The major histocompatibility complex class I gene enhancer binding proteins MBP1 and MBP2 are other representatives of this ZAS protein family. (C) 1996 Academic Press, Inc. |
