| First Author | Mercer RW | Year | 1986 |
| Journal | Mol Cell Biol | Volume | 6 |
| Issue | 11 | Pages | 3884-90 |
| PubMed ID | 3025616 | Mgi Jnum | J:19611 |
| Mgi Id | MGI:67942 | Doi | 10.1128/mcb.6.11.3884 |
| Citation | Mercer RW, et al. (1986) Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells. Mol Cell Biol 6(11):3884-90 |
| abstractText | We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides. |
