| First Author | Swiers G | Year | 2013 |
| Journal | Nat Commun | Volume | 4 |
| Pages | 2924 | PubMed ID | 24326267 |
| Mgi Jnum | J:226222 | Mgi Id | MGI:5696502 |
| Doi | 10.1038/ncomms3924 | Citation | Swiers G, et al. (2013) Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level. Nat Commun 4:2924 |
| abstractText | Haematopoietic stem cells (HSCs) are the founding cells of the adult haematopoietic system, born during ontogeny from a specialized subset of endothelium, the haemogenic endothelium (HE) via an endothelial-to-haematopoietic transition (EHT). Although recently imaged in real time, the underlying mechanism of EHT is still poorly understood. We have generated a Runx1 +23 enhancer-reporter transgenic mouse (23GFP) for the prospective isolation of HE throughout embryonic development. Here we perform functional analysis of over 1,800 and transcriptional analysis of 268 single 23GFP(+) HE cells to explore the onset of EHT at the single-cell level. We show that initiation of the haematopoietic programme occurs in cells still embedded in the endothelial layer, and is accompanied by a previously unrecognized early loss of endothelial potential before HSCs emerge. Our data therefore provide important insights on the timeline of early haematopoietic commitment. |
