| First Author | Brunet JF | Year | 1986 |
| Journal | Nature | Volume | 322 |
| Issue | 6076 | Pages | 268-71 |
| PubMed ID | 3090449 | Mgi Jnum | J:8372 |
| Mgi Id | MGI:56839 | Doi | 10.1038/322268a0 |
| Citation | Brunet JF, et al. (1986) The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14. Nature 322(6076):268-71 |
| abstractText | Classical phenomenological approaches to the study of the mechanism of T-cell-mediated cytotoxicity have now given way to a search for molecules involved in this function; this is attempted either by subcellular and biochemical fractionation of material from cytotoxic cells, or through the characterization of molecules recognized by cytotoxicity-inhibiting monoclonal antibodies Molecules having a role in cytotoxicity may also be identified by detecting the corresponding messenger RNA transcripts. Such an approach may include, as a first step, the search for transcripts as specific as possible to cytotoxic T cells; only secondarily can their actual relevance to cytotoxicity be investigated. We report here the preparation and systematic screening of a differential complementary DNA bank, in which we detected three distinct messenger RNA transcripts (CTLA-1, CTLA-2 and CTLA-3) present in various cytotoxic T cells but not (or less so) in a range of non-cytotoxic lymphoid cells. We describe the co-inducibility of these transcripts and of cytotoxicity in thymocytes and hybridoma cells, the sequence of CTLA-1 cDNA, its protein homology with serine esterases and the localization of the corresponding gene to mouse chromosome 14. |
