| First Author | Choe K | Year | 2022 |
| Journal | Nat Immunol | Volume | 23 |
| Issue | 2 | Pages | 330-340 |
| PubMed ID | 35087231 | Mgi Jnum | J:330431 |
| Mgi Id | MGI:7264833 | Doi | 10.1038/s41590-021-01101-1 |
| Citation | Choe K, et al. (2022) Intravital three-photon microscopy allows visualization over the entire depth of mouse lymph nodes. Nat Immunol 23(2):330-340 |
| abstractText | Intravital confocal microscopy and two-photon microscopy are powerful tools to explore the dynamic behavior of immune cells in mouse lymph nodes (LNs), with penetration depth of ~100 and ~300 mum, respectively. Here, we used intravital three-photon microscopy to visualize the popliteal LN through its entire depth (600-900 mum). We determined the laser average power and pulse energy that caused measurable perturbation in lymphocyte migration. Long-wavelength three-photon imaging within permissible parameters was able to image the entire LN vasculature in vivo and measure CD8(+) T cells and CD4(+) T cell motility in the T cell zone over the entire depth of the LN. We observed that the motility of naive CD4(+) T cells in the T cell zone during lipopolysaccharide-induced inflammation was dependent on depth. As such, intravital three-photon microscopy had the potential to examine immune cell behavior in the deeper regions of the LN in vivo. |
