| First Author | Stevens JL | Year | 2002 |
| Journal | Science | Volume | 296 |
| Issue | 5568 | Pages | 755-8 |
| PubMed ID | 11934987 | Mgi Jnum | J:125417 |
| Mgi Id | MGI:3758505 | Doi | 10.1126/science.1068943 |
| Citation | Stevens JL, et al. (2002) Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit. Science 296(5568):755-8 |
| abstractText | Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein kinase pathway required for vulva development in Caenorhabditis elegans. We generated sur2-/- embryonic stem cells to analyze its function as a mammalian Mediator component. Our results show that Sur2 forms a subcomplex of the Mediator with two other subunits, TRAP/Med100 and 95. Knock-out of Sur2 prevents activation by E1A-CR3 and the mitogen-activated protein kinase-regulated ETS transcription factor Elk-1, but not by multiple other transcription factors. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter by binding to its Sur2 subunit. |
