| First Author | Reese J | Year | 1999 |
| Journal | Mol Cell Endocrinol | Volume | 150 |
| Issue | 1-2 | Pages | 23-31 |
| PubMed ID | 10411296 | Mgi Jnum | J:55394 |
| Mgi Id | MGI:1337901 | Doi | 10.1016/s0303-7207(99)00033-7 |
| Citation | Reese J, et al. (1999) COX-2 compensation in the uterus of COX-1 deficient mice during the pre-implantation period [published erratum appears in Mol Cell Endocrinol 1999 Aug 20;154(1-2):195]. Mol Cell Endocrinol 150(1-2):23-31 |
| abstractText | Prostaglandins (PGs) produced by cyclooxygenase (COX) participate in many aspects of female reproduction. The two isoforms of cyclooxygenase, COX-1 and COX-2, have distinct expression patterns in the mouse uterus during the peri-implantation period and suggest their independent contribution to uterine PGs. Using wild type and COX-1(-/-) mice, we examined the role of COX-1-derived PGs on day 4 of pregnancy, when its expression is maximal. Uterine vascular permeability was measured by 125I-labeled bovine serum albumin (BSA) uptake, and PG content was measured by gas chromatography-mass spectrometry. Vascular permeability and PG concentrations were reduced in COX-1(-/-) mice, but by less than the expected amount. After ovariectomy, uterine vascular permeability declined in both groups, but returned to baseline in wild type and was exaggerated in COX-1(-/-) females after treatment with ovarian steroids. Most importantly, COX-1(-/-) uteri displayed COX-2 expression on the morning of day 4, when COX-2 is normally absent. This hybridization pattern resembles the native expression of COX-1, and may partially offset the loss of COX-1-derived PGs. These data indicate that COX-1-derived PGs are important during uterine preparation for implantation, and that COX-2 compensation occurs in the absence of COX-1. |
