| First Author | Kuroki S | Year | 2015 |
| Journal | Genesis | Volume | 53 |
| Issue | 6 | Pages | 387-93 |
| PubMed ID | 26012873 | Mgi Jnum | J:233557 |
| Mgi Id | MGI:5784964 | Doi | 10.1002/dvg.22863 |
| Citation | Kuroki S, et al. (2015) Development of a general-purpose method for cell purification using Cre/loxP-mediated recombination. Genesis 53(6):387-93 |
| abstractText | A mammalian body is composed of more than 200 different types of cells. The purification of a certain cell type from tissues/organs enables a wide variety of studies. One popular cell purification method is immunological isolation, using antibodies against specific cell surface antigens. However, this is not a general-purpose method, since suitable antigens have not been found in certain cell types, including embryonic gonadal somatic cells and Sertoli cells. To address this issue, we established a knock-in mouse line, named R26 KI, designed to express the human cell surface antigen hCD271 through Cre/loxP-mediated recombination. First, we used the R26 Kl mouse line to purify embryonic gonadal somatic cells. Gonadal somatic cells were purified from the R26 KI; Nr5a1-Cre-transgenic (tg) embryos almost equally as efficiently as from Nr5a1-hCD271-tg embryos. Second, we used the R26 KI mouse line to purify Sertoli cells successfully from R26 KI; Amh-Cre-tg testes. In summary, we propose that the R26 KI mouse line is a powerful tool for the purification of various cell types. |
