Primary Identifier | MGI:3604205 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rassf1 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Transfecting ES cells containing a floxed 1alpha exon with mice expressing cre recombinase resulted in the excision of the exon, thereby ablating isoform 1A expression. RT-PCR of lung confirmed absence of transcript from the exon, while the 1C isoform remained unaffected. |