Primary Identifier | MGI:2136646 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Vhl |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A CMV-hyTK selection cassette flanked by two loxP sites was inserted approximately 2.6kb upstream of exon 1. A third loxP site was inserted into intron 1. ES cells with this mutation were transiently transfected with a vector driving expression of Cre recombinase. ES cells containing only one loxP site, in which the promoter and exon 1 were deleted, were selected. These cells were injected into blastocyts to generate a the allele designated Vhltm1.1Jae. |