Primary Identifier | MGI:7544867 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mirc31 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector containing a loxP flanked PGK-EM7-NeobpA cassette replaced Mirlet7c-2 and Mirlet7b which make up the Mirc31 cluster. Cre-mediated recombination removed the neo cassette. PCR sequencing confirmed the presence of the 801-bp genomic deletion of Mirlet7b and Mirlet7c-2. |