| First Author | Guardiola-Serrano F | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 376 |
| Issue | 4 | Pages | 748-52 |
| PubMed ID | 18814840 | Mgi Jnum | J:143380 |
| Mgi Id | MGI:3826754 | Doi | 10.1016/j.bbrc.2008.09.070 |
| Citation | Guardiola-Serrano F, et al. (2008) Gene trapping identifies a putative tumor suppressor and a new inducer of cell migration. Biochem Biophys Res Commun 376(4):748-52 |
| abstractText | Tumor necrosis factor alpha (TNFalpha) is a pleiotropic cytokine involved in apoptotic cell death, cellular proliferation, differentiation, inflammation, and tumorigenesis. In tumors it is secreted by tumor associated macrophages and can have both pro- and anti-tumorigenic effects. To identify genes regulated by TNFalpha, we performed a gene trap screen in the mammary carcinoma cell line MCF-7 and recovered 64 unique, TNFalpha-induced gene trap integration sites. Among these were the genes coding for the zinc finger protein ZC3H10 and for the transcription factor grainyhead-like 3 (GRHL3). In line with the dual effects of TNFalpha on tumorigenesis, we found that ZC3H10 inhibits anchorage independent growth in soft agar suggesting a tumor suppressor function, whereas GRHL3 strongly stimulated the migration of endothelial cells which is consistent with an angiogenic, pro-tumorigenic function. |
