| First Author | Corr M | Year | 1997 |
| Journal | J Immunol | Volume | 159 |
| Issue | 10 | Pages | 4999-5004 |
| PubMed ID | 9366427 | Mgi Jnum | J:44066 |
| Mgi Id | MGI:1099317 | Doi | 10.4049/jimmunol.159.10.4999 |
| Citation | Corr M, et al. (1997) Costimulation provided by DNA immunization enhances antitumor immunity. J Immunol 159(10):4999-5004 |
| abstractText | The interaction of the TCR with MHC class I-bound Ag is insufficient for the priming of CTL unless secondary costimulatory signals are provided. To ascertain the minimum elements required to activate an Ag-specific CTL response in vivo, we injected mice intradermally or i.m. with plasmid DNA encoding a MHC class I-restricted peptide Ag (minigene) and different membrane-bound costimulatory ligands. The minigene-encoded epitope only primed a specific CTL response if injected in the vicinity of an ectopically expressed costimulatory ligand. Vector encoding B7-1 was repeatedly more potent at stimulating a cytolytic response than vector encoding B7-2. In contrast the B7-2-encoding plasmid preferentially enhanced Ag-specific Ab responses when injected with either protein or a cDNA expression vector. Gene vaccination with plasmids encoding OVA and B7-1, but not B7-2, prolonged survival in mice challenged with an OVA-transfected tumor. These results show that functional B7-1 transfection can be achieved in vivo and induces the selective induction of CTL. The data suggest that B7-1 plasmids should be coadministered with naked DNA vaccines that aim to induce tumor-specific cellular immunity. |
