| First Author | Abdullah A | Year | 2022 |
| Journal | Neurochem Res | Volume | 47 |
| Issue | 9 | Pages | 2793-2804 |
| PubMed ID | 35753011 | Mgi Jnum | J:329260 |
| Mgi Id | MGI:7341989 | Doi | 10.1007/s11064-022-03651-8 |
| Citation | Abdullah A, et al. (2022) Fut9 Deficiency Causes Abnormal Neural Development in the Mouse Cerebral Cortex and Retina. Neurochem Res 47(9):2793-2804 |
| abstractText | alpha1,3-Fucosyltransferase 9 (Fut9) is responsible for the synthesis of Lewis X [Le(X), Galbeta1-4(Fucalpha1-3)GlcNAc] carbohydrate epitope, a marker for pluripotent or multipotent tissue-specific stem cells. Although Fut9-deficient mice show anxiety-related behaviors, structural and cellular abnormalities in the brain remain to be investigated. In this study, using in situ hybridization and immunohistochemical techniques in combination, we clarified the spatiotemporal expression of Fut9, together with Le(X), in the brain and retina. We found that Fut9-expressing cells are positive for Ctip2, a marker of neurons residing in layer V/VI, and TLE4, a marker of corticothalamic projection neurons (CThPNs) in layer VI, of the cortex. A birthdating analysis using 5-ethynyl-2'-deoxyuridine at embryonic day (E)11.5, 5-bromo-2'-deoxyuridine at E12.5, and in utero electroporation of a GFP expression plasmid at E14.5 revealed a reduction in the percentage of neurons produced at E11.5 in layer VI/subplate of the cortex and in the ganglion cell layer of the retina in P0 Fut9(-/-) mice. Furthermore, this reduction in layer VI/subplate neurons persisted into adulthood, leading to a reduction in the number of Ctip2(strong)/Satb2(-) excitatory neurons in layer V/VI of the adult Fut9(-/-) cortex. These results suggest that Fut9 plays significant roles in the differentiation, migration, and maturation of neural precursor cells in the cortex and retina. |
