| First Author | Grégoire C | Year | 2007 |
| Journal | Eur J Immunol | Volume | 37 |
| Issue | 11 | Pages | 3259-69 |
| PubMed ID | 17918199 | Mgi Jnum | J:126321 |
| Mgi Id | MGI:3761031 | Doi | 10.1002/eji.200737563 |
| Citation | Gregoire C, et al. (2007) Deletion of the LIME adaptor protein minimally affects T and B cell development and function. Eur J Immunol 37(11):3259-69 |
| abstractText | LIME (Lck-interacting membrane protein) is a transmembrane adaptor that associates with the Lck and Fyn protein tyrosine kinases and with the C-terminal Src kinase (Csk). To delineate the role of LIME in vivo, LIME-deficient mice were generated. Although Lime transcripts were expressed in immature and mature B and T cells, the absence of LIME impeded neither the development nor the function of B and T cells. TCR transgenic mice deprived of LIME showed, however, a 1.8-fold enhancement in positive selection. Since B cells and activated T cells express LIME and the related adaptor NTAL, mice lacking both adaptors were generated. Double-deficient mice showed no defect in the development and function of B and T cells, and the lack of LIME had no effect on the autoimmune syndrome that develops in aged NTAL-deficient mice. In contrast to a previous report, we further showed that this autoimmune syndrome develops in the absence of T cells. Therefore, our in vivo results refute all the previous roles postulated for LIME on the basis of studies of transformed B and T cells and demonstrate that LIME has no seminal role in the signaling cassette operated by antigen receptors and coreceptors. |
