Primary Identifier | MGI:3613519 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Aco1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The floxed exon 3 was removed from Aco1tm1.1Mwh by mating with mice expressing Tg(Pgk1-cre)1Lni, creating a null allele. Removal of exon 3 creates a frameshift with premature termination of translation in exon 4. Western blotting confirmed absence of protein in kidney, duodenum, liver and spleen extracts. |