| First Author | Enver T | Year | 1994 |
| Journal | Dev Biol | Volume | 165 |
| Issue | 2 | Pages | 574-84 |
| PubMed ID | 7958423 | Mgi Jnum | J:21112 |
| Mgi Id | MGI:69157 | Doi | 10.1006/dbio.1994.1277 |
| Citation | Enver T, et al. (1994) Analysis of the developmental and transcriptional potentiation functions of 5'HS2 of the murine beta-globin locus control region in transgenic mice. Dev Biol 165(2):574-84 |
| abstractText | We analyze the role of 5'HS2 of the mouse beta-globin LCR in the transcriptional and developmental regulation of beta-globin gene expression. Previous studies have shown that the human beta-globin gene behaves as an adult gene in transgenic mice, being expressed in fetal liver and bone marrow-derived erythroblasts but not in yolk sac-derived embryonic erythroid cells. We show that linkage of mLCR5'HS2 to a human beta-globin gene alters this pattern of expression during ontogeny, resulting in expression of the linked beta-globin gene at all stages of murine erythroid development. Expression was independent of integration position and correlated with transgene copy number. Our results provide the first test of a phylogenetically homologous LCR in transgenic mice and demonstrate evolutionary conservation of both developmental and transcriptional potentiation functions between mammalian beta-globin LCRs. |
