First Author | Wohl SG | Year | 2016 |
Journal | Sci Rep | Volume | 6 |
Pages | 35423 | PubMed ID | 27739496 |
Mgi Jnum | J:259983 | Mgi Id | MGI:6102038 |
Doi | 10.1038/srep35423 | Citation | Wohl SG, et al. (2016) The microRNA expression profile of mouse Muller glia in vivo and in vitro. Sci Rep 6:35423 |
abstractText | The profile of miRNAs in mature glia is not well characterized, and most studies have been done in cultured glia. In order to identify the miRNAs in adult and young (postnatal day 11/12) Muller glia of the neural retina, we isolated the Muller glia from Rlbp-CreER: Stop(f/f)-tdTomato mice by means of fluorescent activated cell sorting and analyzed their miRNAs using NanoStrings Technologies((R)). In freshly isolated adult Muller glia, we identified 7 miRNAs with high expression levels in the glia, but very low levels in the retinal neurons. These include miR-204, miR-9, and miR-125-5p. We also found 15 miRNAs with high levels of expression in both neurons and glia, and many miRNAs that were enriched in neurons and expressed at lower levels in Muller glia, such as miR-124. We next compared miRNA expression of acutely isolated Muller glia with those that were maintained in dissociated culture for 8 and 14 days. We found that most miRNAs declined in vitro. Interestingly, some miRNAs that were not highly expressed in adult Muller glia increased in cultured cells. Our results thus show the miRNA profile of adult Muller glia and the effects of cell culture on their levels. |