First Author | Senftleben U | Year | 2001 |
Journal | Immunity | Volume | 14 |
Issue | 3 | Pages | 217-30 |
PubMed ID | 11290332 | Mgi Jnum | J:68347 |
Mgi Id | MGI:1932592 | Doi | 10.1016/s1074-7613(01)00104-2 |
Citation | Senftleben U, et al. (2001) IKKbeta is essential for protecting T cells from TNFalpha-induced apoptosis. Immunity 14(3):217-30 |
abstractText | Transcription factor NF-kappaB, whose activation depends on the IKKbeta catalytic subunit of the IkappaB kinase, was assigned with both anti- and proapoptotic functions in T lymphocytes. To critically evaluate these functions, we transferred Ikkbeta-/- or wild-type (wt) fetal liver (FL) stem cells into lethally irradiated mice. Ikkbeta-/- radiation chimeras show thymic rudiments, aberrant lymphoid organs, and absence of T cells. T lymphopoiesis is rescued when Ikkbeta-/- stem cells are cotransferred with wt bone marrow, suggesting that IKKbeta may mediate its lymphopoietic function via extrinsic factors. However, almost normal development of Ikkbeta-/- T cells is observed upon removal of type 1 TNFalpha receptor, indicating that TNFalpha signaling accounts for the absence of Ikkbeta-/- T cells. Indeed, Ikkbeta-/- radiation chimeras exibit elevated circulating TNFalpha, and Ikkbeta-/- thymocytes display increased TNFalpha sensitivity. |