| Primary Identifier | MGI:5620879 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Dact1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | J:211189 used HEPD0545_2_A04 mutant ES cells to create this allele. |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 71359010 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 71359851. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a reporter knockout mouse. |
