| First Author | Qiao Y | Year | 2012 |
| Journal | FEBS Lett | Volume | 586 |
| Issue | 7 | Pages | 1022-6 |
| PubMed ID | 22569257 | Mgi Jnum | J:184513 |
| Mgi Id | MGI:5424269 | Doi | 10.1016/j.febslet.2012.02.045 |
| Citation | Qiao Y, et al. (2012) TLR-induced NF-kappaB activation regulates NLRP3 expression in murine macrophages. FEBS Lett 586(7):1022-6 |
| abstractText | NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1beta. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-kappaB dependent manner. Furthermore, the corresponding NF-kappaB binding sites (nt -1303 to -1292 and -1238 to -1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-kappaB binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3. |
