| Primary Identifier | MGI:6111536 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Gk5 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A G>A point mutation was introduced using the CRISPR/Cas9 system and sgRNA 5'-CTCCAAACATGGCCGACTGC-3'. The point mutation in the kinase domain changes aspartic acid codon 280 to an asparagine codon (p.Asp280Asn or p.D280N) and creates a kinase-dead allele. Glycerol kinase activity assays showed kinase activity reduced to less than 10% of wild-type. |
