| First Author | Means GD | Year | 2000 |
| Journal | Genomics | Volume | 65 |
| Issue | 3 | Pages | 213-23 |
| PubMed ID | 10857745 | Mgi Jnum | J:62168 |
| Mgi Id | MGI:1858533 | Doi | 10.1006/geno.2000.6173 |
| Citation | Means GD, et al. (2000) A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation. Genomics 65(3):213-23 |
| abstractText | A physical clone contig has been constructed, spanning 2 Mb on the proximal mouse X chromosome containing the mouse scurfy (sf) and tattered (Td) mutations. Extensive transcript mapping in this interval has identified 37 potential transcription units, including a number of novel genes, and 4 pseudogenes. These genes have been ordered by STS content and restriction mapping. Comparison of the transcript map to the corresponding region in human Xp11.23-p11.22 shows extensive homology, with complete conservation of gene order for loci in common between the two maps. Further, using a novel method to identify simple sequence length polymorphisms, we have developed a number of genetic markers, which has enabled the region containing the sf mutation to be narrowed to <300 kb. This contig has already allowed the cloning of the Td gene using a candidate gene approach and now serves as a starting point for the cloning of the sf mutation. |
